Two cases of zoonotic cryptosporidiosis in Spain by the unusual species Cryptosporidium ubiquitum and Cryptosporidium felis.

INTRODUCTION Two cases of infection by zoonotic transmission of unusual species of Cryptosporidium were detected in 2010-2011 in Spain (León and Zaragoza). MATERIALS AND METHODS Cryptosporidium spp. was detected by microscopic examination of modified Ziehl-Neelsen stained fecal smears. PCR-RFLP of the SSUrDNA gene and sequencing of the amplified fragment confirmed the species. RESULTS C. ubiquitum and C. felis were identified in samples from an immunocompetent child and from a HIV-positive adult, respectively. CONCLUSIONS This is the first report of human infection by C. ubiquitum (cervine) and autochthonous C. felis, identified in Spain.